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Solved 2024: BOTANY: Mushroom Cultivation (BOTSEC-151T)<br>FYUG Even Semester Exam, 2024

Course No: BOTSEC-151T | Full Marks: 50 | Pass Marks: 20 | Time: 2 Hours

Subject: Botany (Skill Enhancement Course)

Semester: 2nd Semester

Exam Name: FYUG Even Semester Exam, 2024


SECTION-A (Answer any fifteen) 1 × 15 = 15

1. Define mushrooms.

Mushrooms are the macroscopic fruiting bodies of certain fungi, typically belonging to the Basidiomycota or Ascomycota divisions, which can be either edible or poisonous

.

2. What do you mean by 'toadstools'?

The term 'toadstools' is commonly used to refer to poisonous or inedible mushrooms, often characterized by bright colors or umbrella-like caps

.

3. What is button mushroom?

Button mushroom refers to Agaricus bisporus, the most widely cultivated and consumed mushroom globally, known for its white, rounded cap

.

4. What is oyster mushroom?

Oyster mushroom refers to species of Pleurotus, named for their oyster-shaped caps and rapid growth on various lignocellulosic substrates

.

5. What is inoculation hook?

An inoculation hook is a laboratory tool with a bent metal tip used to transfer small pieces of fungal mycelium or agar into growth media

.

6. Define inoculation loop.

An inoculation loop consists of a handle with a thin wire forming a small loop at the end, used to pick up and transfer microbial cultures or spores

.

7. Define culture rack.

A culture rack is a tiered structure used in laboratory or cultivation rooms to organize and hold Petri dishes, flasks, or spawn bottles during incubation

.

8. Write about low-cost stove.

A low-cost stove is a simple heating device used in small-scale mushroom units for sterilizing substrates or boiling grains for spawn preparation

.

9. What is mushroom bed?

A mushroom bed is a prepared surface or container filled with sterilized or pasteurized substrate where fungal mycelium is grown for fruiting

.

10. Define composting.

Composting is the biological process of decomposing organic matter (like straw and manure) by microbes to create a nutrient-rich substrate suitable for button mushrooms

.

11. Write about maize straw for bed preparation.

Maize straw serves as a locally available lignocellulosic substrate that can be chopped and soaked to prepare beds for oyster mushroom cultivation

.

12. What do you mean by low-cost technology?

Low-cost technology involves using inexpensive, locally available materials and manual methods to cultivate mushrooms without high investment in heavy machinery

.

13. Define storage of mushrooms.

Storage refers to the techniques used to preserve the shelf life of harvested mushrooms, preventing spoilage due to their high moisture content

.

14. What is canning?

Canning is a long-term preservation method where mushrooms are cleaned, blanched, and sealed in airtight cans with brine, followed by heat sterilization

.

15. Name two amino acids present in mushrooms.

Lysine and Tryptophan

.

16. Name some mineral elements present in mushrooms.

Potassium, Phosphorus, Magnesium, and Selenium

.

17. Name a mushroom toxin.

α-Amanitin (found in Amanita phalloides)

.

18. Name one mushroom research centre at National level.

ICAR-Directorate of Mushroom Research (DMR), Solan, Himachal Pradesh.

19. Name one mushroom research centre at regional level.

Mushroom Development Foundation (MDF), Guwahati (Regional example for NE India)

.

20. Why is mushroom regarded as an ideal food for diabetic patients?

Mushrooms are ideal because they are low in calories, low in sugar/starch, and contain no cholesterol, helping maintain stable blood glucose levels

.

SECTION-B (Answer any five) 2 × 5 = 10

21. Write a note on nutritional value of edible mushrooms. [2]

Edible mushrooms are rich in high-quality proteins (20-35% dry weight), dietary fiber, and essential vitamins (B-complex, C, and D)

. They are low in fats and contain essential minerals like Potassium and Selenium.

23. locally available substrates for mushroom cultivation. [2]

Commonly used local substrates include:

  • Paddy straw (most common for Oyster and Straw mushrooms).
  • Maize straw and wheat straw.
  • Dried banana leaves and sugarcane bagasse.
  • 25. Give a brief account of bed preparation with paddy straw. [2]

    Paddy straw is soaked in water (sometimes with lime) for 12-24 hours, drained to maintain 65% moisture, and then layered in blocks or bags while mixing in mushroom spawn

    .

    27. Write about mushroom drying. [2]

    Drying involves removing moisture either by sun-drying or using mechanical dehydrators

    . It reduces the water activity, allowing mushrooms to be stored for several months without spoilage.

    29. Write a note on cost-benefit ratio of mushroom cultivation. [2]

    Mushroom cultivation is highly profitable with a low gestation period

    . The cost-benefit ratio is favorable because it uses agricultural waste as substrate and requires minimal land space.

    SECTION-C (Answer any five) 5 × 5 = 25

    32. Write an essay on Agaricus bisporus. [5]

    Introduction: Agaricus bisporus, commonly known as the White Button Mushroom, is the most commercially cultivated species worldwide

    .

    Cultivation Steps:

  • Composting: Preparing a specific substrate using straw, horse manure, and gypsum.
  • Spawning: Inoculating the compost with pure mushroom culture (spawn).
  • Casing: Covering the colonized compost with a layer of soil/peat to induce fruiting.
  • Cropping: Harvesting the mushrooms at the "button" stage.
  • 34. Describe the preparation of spawn in detail. [5]

    Spawn is the "seed" of the mushroom

    . Its preparation involves:
  • Grain Selection: Using wheat, sorghum, or pearl millet grains.
  • Boiling and Sterilization: Grains are boiled, mixed with Calcium Carbonate, and autoclaved at 121°C.
  • Inoculation: Pure culture of the desired mushroom is added to the grains under sterile conditions.
  • Incubation: Kept in a dark room until the mycelium completely covers the grains.
  • 36. Describe mushroom composting. [5]

    Composting creates a selective substrate for Agaricus. It is done in two phases:

  • Phase I (Outdoor): Raw materials like straw and poultry manure are wetted and piled. Microbes begin fermentation, raising temperatures to 70°C.
  • Phase II (Indoor/Pasteurization): The compost is heat-treated to kill pests and pathogens while converting ammonia into microbial protein.
  • 38. Describe crude fibre and vitamins in mushrooms. [3+2=5]

    Crude Fibre: Mushrooms contain significant amounts of chitin and hemicellulose in their cell walls, acting as dietary fiber that aids digestion and prevents constipation

    .

    Vitamins: They are excellent sources of Vitamin B-complex (Riboflavin, Niacin) and Vitamin D

    . Some species also provide Vitamin C and Folate.

    39. Give a detailed account of various types of food prepared from mushrooms. [5]

    Mushrooms are versatile in the kitchen

    :
  • Fresh: Used in soups, stir-fries, and curries.
  • Processed: Mushroom powder (used in biscuits/health drinks), mushroom pickles, and ketchup.
  • Preserved: Canned mushrooms and sun-dried mushrooms for off-season use.
  • Exam Focus Enhancements

  • Exam Tips: For "Mushroom Bed Preparation" (Q35), always mention moisture control (approx. 65%) as a critical internal factor.
  • Common Mistakes: Do not confuse "Inoculation Hook" (bent tip) with "Inoculation Loop" (circular tip).
  • Important Formulas: Cost-Benefit Ratio = Total Returns / Total Costs.
  • Strategy: Use "Agaricus bisporus" as your primary example for any question regarding commercial cultivation.
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