Practical 2: Chordates

Table of Contents

1. Study of Protochordate Museum Specimens

This "spotting" exercise involves identifying key protochordate specimens.

Subphylum Specimen Example Key Identification Points (Comments)
Hemichordata Balanoglossus (Acorn Worm) 1. Soft, elongated, worm-like body. 2. Body is divided into three distinct regions: an anterior proboscis (acorn-shaped), a short collar, and a long trunk.
Urochordata Herdmania (Sea Squirt) 1. Bag-like or "purse-like" body, attached to a substratum. 2. Body is covered by a leathery tunic or test. 3. Two siphons are visible: a branchial siphon (incurrent) and an atrial siphon (excurrent).
Cephalochordata Amphioxus (Lancelet) 1. Small, fish-like, translucent body, pointed at both ends. 2. Shows segmented muscle blocks (myotomes). 3. Anterior end has an oral hood with tentacles (oral cirri).

2. Study of Protochordate Sections (Slides)

This exercise involves identifying key histological sections of *Balanoglossus* and *Amphioxus*.

Specimen (Section) Key Identification Points (Draw and Label)
Balanoglossus T.S. Proboscis 1. Circular outline. 2. Epidermis on the outside. 3. A central proboscis coelom. 4. Presence of a glomerulus (excretory organ) and a central complex.
Balanoglossus T.S. Branchiogenital Region 1. Shows the dorsal pharynx with gill slits. 2. Shows the "wings" of the coelom containing the gonads (testes or ovaries). 3. Ventral esophagus is present.
Amphioxus T.S. Pharyngeal Region 1. A large, central pharynx with numerous diagonal gill bars. 2. Dorsally located dorsal nerve cord (hollow) and notochord (solid rod). 3. V-shaped myotomes (muscle segments). 4. Atrium space around the pharynx.
Amphioxus T.S. Intestinal Region 1. A simple, oval-shaped intestine (midgut) in the center. 2. Notochord and dorsal nerve cord are present dorsally. 3. Myotomes are present. 4. No gill bars.
Amphioxus T.S. Caudal Region 1. Section through the tail. 2. Shows the caudal fin. 3. Contains notochord, dorsal nerve cord, and myotomes, but no intestine or pharynx.

3. Study of Vertebrate Museum Specimens

This is a "spotting" exercise for identifying specimens from the major vertebrate classes.

Class Specimen Example Key Identification Points (Comments)
Agnatha Petromyzon (Lamprey) 1. Eel-like, scaleless body. 2. Jaws are absent. 3. Possesses a circular, suctorial mouth (buccal funnel) armed with horny teeth. 4. Shows 7 pairs of gill slits.
Chondrichthyes Scoliodon (Shark) 1. Cartilaginous fish with a spindle-shaped body. 2. Ventral, crescent-shaped mouth. 3. 5-7 pairs of gill slits, not covered by an operculum. 4. Skin is covered with placoid scales (tooth-like). 5. Heterocercal tail (unequal lobes).
Osteichthyes Labeo (Rohu / Bony Fish) 1. Bony fish with a spindle-shaped body. 2. Terminal mouth. 3. 4 pairs of gills covered by a bony flap called an operculum. 4. Skin is covered with cycloid/ctenoid scales. 5. Homocercal tail (equal lobes).
Amphibia Rana (Frog) 1. Soft, moist, glandular skin. 2. Body divided into head and trunk (neck and tail absent in adult). 3. Limbs are pentadactyl; hind limbs are large and webbed for jumping and swimming.
Reptilia Calotes (Garden Lizard) 1. Body covered with dry, horny epidermal scales. 2. Body divided into head, neck, trunk, and tail. 3. Claws are present on the digits.
Reptilia Naja (Cobra) 1. Limbless, snake-like body covered in scales. 2. Presence of a hood behind the head. 3. Poisonous (neurotoxic).
Aves Columba (Pigeon) 1. Body covered with feathers. 2. Forelimbs are modified into wings for flight. 3. Jaws are modified into a beak (teeth are absent).
Mammalia Pteropus (Bat) 1. Body covered with fur (hair). 2. Forelimbs are modified into wings for true flight; the "wing" is a thin membrane of skin (patagium) stretched between elongated fingers. 3. Presence of external ears (pinnae).

4. Key for Identification of Poisonous and Non-poisonous Snakes

This exercise involves distinguishing between poisonous and non-poisonous snakes based on key morphological features.

Feature Poisonous Snake Non-Poisonous Snake
Ventral Scales (Belly Scales) Small and uniform OR Broad, but not full width (e.g., Sea Snakes).
Exception: Vipers and Cobras/Kraits have broad ventrals that cover the full belly.
Almost always broad and cover the full width of the belly.
Head Scales Usually small and irregular (e.g., Vipers).
Exception: Cobras and Kraits have large head shields.
Usually large and regular (head shields), like in a Rat Snake.
Loreal Pit Present in Pit Vipers (a heat-sensing pit between the eye and nostril). Absent.
Tail Shape Laterally compressed or "flat" like an oar (in Sea Snakes). Usually cylindrical and tapering.
Fangs (if visible) Presence of enlarged, grooved or hollow teeth (fangs) in the upper jaw. All teeth are usually small and uniform.
Bite Mark Two distinct fang marks, often with a row of smaller teeth. A 'U' shaped series of small, uniform teeth marks.
Exam Tip: In India, the "Big Four" poisonous snakes are: Cobra (hood), Krait (large hexagonal scales on back), Russell's Viper (triangular head, chain-like spots), and Saw-scaled Viper (small, triangular head, arrow mark).

5. Study of Birds (Specimens, Beaks, and Claws)

A. Six Common Birds

You may be asked to identify and state the order of 6 common birds. Examples:

  1. House Sparrow (*Passer domesticus*) - Order: Passeriformes
  2. House Crow (*Corvus splendens*) - Order: Passeriformes
  3. Pigeon (*Columba livia*) - Order: Columbiformes
  4. Parrot (*Psittacula krameri*) - Order: Psittaciformes
  5. Domestic Fowl (*Gallus gallus*) - Order: Galliformes
  6. Duck (*Anas sp.*) - Order: Anseriformes

B. Types of Beaks and Claws

Observe specimens or charts to understand how beak and claw shapes are adapted to diet and habitat.

Bird Beaks

Beak Type Shape Adaptation (Diet) Example
Grain-eating Short, conical, strong. Crushing seeds. Sparrow, Pigeon
Fruit-eating Hooked, sharp, strong. Tearing fruits. Parrot
Flesh-eating Short, strong, sharp, hooked. Tearing flesh. Eagle, Vulture
Insect-eating Thin, slender, pointed. Picking insects. Robin, Hoopoe
Filtering Broad, flat, with filters. Filtering mud for food. Duck
[Image of types of bird beaks]

Bird Claws (Feet)

Claw Type Shape Adaptation (Habitat/Action) Example
Perching Three toes forward, one backward (slender). Grasping branches. Sparrow, Crow
Grasping (Raptorial) Large, hooked, sharp talons. Catching and killing prey. Eagle, Hawk
Scratching Strong, short, thick toes with blunt claws. Scratching the ground for food. Fowl, Chicken
Swimming Toes are connected by a web of skin. Paddling in water. Duck, Goose
Wading Very long, slender, spread-out toes. Walking on soft mud or water plants. Heron, Jacana
[Image of types of bird claws]

6. Mounts from Fowl Head (Pecten and Weberian Ossicles)

Syllabus Note: The syllabus specifies "Mount of Weberian ossicles, pecten from Fowl head". The Pecten is a structure in the bird's eye. However, Weberian ossicles are a chain of small bones found in fish (like carp and catfish), connecting the swim bladder to the inner ear for sound amplification. They are not found in fowl. This is likely a syllabus error. You should prepare to identify the Pecten from a bird and (separately) the Weberian ossicles from a fish. Please clarify this with your instructor.

A. Pecten (from Fowl Eye)

B. Weberian Ossicles (from Fish)